Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.
Identifieur interne : 002D66 ( Main/Exploration ); précédent : 002D65; suivant : 002D67Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.
Auteurs : H-Q Zheng [République populaire de Chine] ; Q. Zhang ; H-X Li ; S-Z Lin ; X-M An ; Z-Y ZhangSource :
- Plant biology (Stuttgart, Germany) [ 1438-8677 ] ; 2011.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Données de séquences moléculaires (MeSH), Populus (métabolisme), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Séquence conservée (MeSH), Séquence d'acides aminés (MeSH), Tabac (composition chimique), Tabac (génétique), Tabac (métabolisme), Tabac (virologie), Triploïdie (MeSH), Virus de la mosaïque du tabac (physiologie).
- MESH :
- composition chimique : Protéines végétales, Tabac.
- génétique : Protéines végétales, Tabac.
- métabolisme : Populus, Protéines végétales, Tabac.
- physiologie : Virus de la mosaïque du tabac.
- virologie : Tabac.
- Alignement de séquences, Données de séquences moléculaires, Régulation de l'expression des gènes végétaux, Séquence conservée, Séquence d'acides aminés, Triploïdie.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Conserved Sequence (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (metabolism), Sequence Alignment (MeSH), Tobacco (chemistry), Tobacco (genetics), Tobacco (metabolism), Tobacco (virology), Tobacco Mosaic Virus (physiology), Triploidy (MeSH).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- chemistry : Tobacco.
- genetics : Tobacco.
- metabolism : Populus, Tobacco.
- physiology : Tobacco Mosaic Virus.
- virology : Tobacco.
- Amino Acid Sequence, Conserved Sequence, Gene Expression Regulation, Plant, Molecular Sequence Data, Sequence Alignment, Triploidy.
Abstract
A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.
DOI: 10.1111/j.1438-8677.2010.00327.x
PubMed: 21143735
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Zhang</name></author><author><name sortKey="Li, H X" sort="Li, H X" uniqKey="Li H" first="H-X" last="Li">H-X Li</name></author><author><name sortKey="Lin, S Z" sort="Lin, S Z" uniqKey="Lin S" first="S-Z" last="Lin">S-Z Lin</name></author><author><name sortKey="An, X M" sort="An, X M" uniqKey="An X" first="X-M" last="An">X-M An</name></author><author><name sortKey="Zhang, Z Y" sort="Zhang, Z Y" uniqKey="Zhang Z" first="Z-Y" last="Zhang">Z-Y Zhang</name></author></analytic><series><title level="j">Plant biology (Stuttgart, Germany)</title><idno type="eISSN">1438-8677</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Conserved Sequence (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Tobacco (chemistry)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Tobacco (virology)</term><term>Tobacco Mosaic Virus (physiology)</term><term>Triploidy (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Populus (métabolisme)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence conservée (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Tabac (composition chimique)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Tabac (virologie)</term><term>Triploïdie (MeSH)</term><term>Virus de la mosaïque du tabac (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la mosaïque du tabac</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Tobacco Mosaic Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Conserved Sequence</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Triploidy</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence conservée</term><term>Séquence d'acides aminés</term><term>Triploïdie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21143735</PMID><DateCompleted><Year>2011</Year><Month>04</Month><Day>21</Day></DateCompleted><DateRevised><Year>2010</Year><Month>12</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1438-8677</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant biology (Stuttgart, Germany)</Title><ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation></Journal><ArticleTitle>Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.</ArticleTitle><Pagination><MedlinePgn>145-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1438-8677.2010.00327.x</ELocationID><Abstract><AbstractText>A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.</AbstractText><CopyrightInformation>© 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>H-Q</ForeName><Initials>HQ</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Q</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>H-X</ForeName><Initials>HX</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>S-Z</ForeName><Initials>SZ</Initials></Author><Author ValidYN="Y"><LastName>An</LastName><ForeName>X-M</ForeName><Initials>XM</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Z-Y</ForeName><Initials>ZY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>11</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biol (Stuttg)</MedlineTA><NlmUniqueID>101148926</NlmUniqueID><ISSNLinking>1435-8603</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014027" MajorTopicYN="N">Tobacco Mosaic Virus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057885" MajorTopicYN="N">Triploidy</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21143735</ArticleId><ArticleId IdType="doi">10.1111/j.1438-8677.2010.00327.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="An, X M" sort="An, X M" uniqKey="An X" first="X-M" last="An">X-M An</name><name sortKey="Li, H X" sort="Li, H X" uniqKey="Li H" first="H-X" last="Li">H-X Li</name><name sortKey="Lin, S Z" sort="Lin, S Z" uniqKey="Lin S" first="S-Z" last="Lin">S-Z Lin</name><name sortKey="Zhang, Q" sort="Zhang, Q" uniqKey="Zhang Q" first="Q" last="Zhang">Q. Zhang</name><name sortKey="Zhang, Z Y" sort="Zhang, Z Y" uniqKey="Zhang Z" first="Z-Y" last="Zhang">Z-Y Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zheng, H Q" sort="Zheng, H Q" uniqKey="Zheng H" first="H-Q" last="Zheng">H-Q Zheng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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